Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.129273 |
Chromosome: | chromosome_16 |
Location: | 5084595 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre16.g684065 | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | AACACCAGGTTTGTCATAGCCACACAAGCA |
Internal bar code: | GGCGGAGTCACCGGGGACCTTA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 761 |
LEAP-Seq percent confirming: | 97.6687 |
LEAP-Seq n confirming: | 1592 |
LEAP-Seq n nonconfirming: | 38 |
LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCCTAAGAGCAGAGAGGGGT |
Suggested primer 2: | CCTGGTTCCAACACACACAC |