| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.129307 |
| Chromosome: | chromosome 8 |
| Location: | 675013 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g360050 | DUR1 | Urea carboxylase/allophanate hydrolase; (1 of 1) 6.3.4.6 - Urea carboxylase / Urease (ATP-hydrolyzing) | 5'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGCAGCAGTTGGCCTTTGCAGTGCCGAC |
| Internal bar code: | TCGCGCATTCCCCAAGCCGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1148 |
| LEAP-Seq percent confirming: | 99.696 |
| LEAP-Seq n confirming: | 656 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGACCACCGGAACTTGTTT |
| Suggested primer 2: | AATAAAGAACCCCATTCCGC |