| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.129326 |
| Chromosome: | chromosome 6 |
| Location: | 5121634 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g281200 | (1 of 1) K16743 - abnormal spindle-like microcephaly-associated protein (ASPM, ASP) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCAGCCGGCGGTGCGGCACAAGTGGGAG |
| Internal bar code: | ACAGCGGCGCGGCTGCTCACTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 314 |
| LEAP-Seq percent confirming: | 98.9039 |
| LEAP-Seq n confirming: | 6226 |
| LEAP-Seq n nonconfirming: | 69 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGCTATTTGGCAGCTACG |
| Suggested primer 2: | CTCTAAAGCGTCGCGCTAAT |