| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.129355 |
| Chromosome: | chromosome 6 |
| Location: | 8095305 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g304650 | PIK3 | Phosphatidylinositol 3-kinase; (1 of 1) PTHR10048:SF22 - PHOSPHATIDYLINOSITOL 4-KINASE BETA | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGGCCTGTGGGCTCAAGGATTGGTGCGG |
| Internal bar code: | GGTGTAGGACTTGTGGATTTAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 272 |
| LEAP-Seq percent confirming: | 97.7686 |
| LEAP-Seq n confirming: | 3067 |
| LEAP-Seq n nonconfirming: | 70 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGCTTGTCTTTGTCCTTGT |
| Suggested primer 2: | ACTGCGCGCTCATATCTTTT |