| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.129367 |
| Chromosome: | chromosome 2 |
| Location: | 7280483 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g145550 | (1 of 1) PTHR13144//PTHR13144:SF0 - TEX261 PROTEIN // PROTEIN TEX261 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAGCCCGTACTAGCCTGCCTCAGCCCATA |
| Internal bar code: | CTTGGGGGTTAAAATAGCCCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 309 |
| LEAP-Seq percent confirming: | 99.6016 |
| LEAP-Seq n confirming: | 1000 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCGATACCTGTTGGCTGTC |
| Suggested primer 2: | GGTGGGGGTTAGTTTACGGT |