Insertion junction: LMJ.RY0402.129370_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.129370
Chromosome:chromosome 2
Location:5511817
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g108300 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGGAAGGAGAAGGAGGACGACACTGATGA
Internal bar code:TTCGCACCACCGGCTATTTTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:1196
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:AACACCTTGGTAAACACGCC
Suggested primer 2:TGGAGGTGGAGTGTGGTGTA