| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.129374 |
| Chromosome: | chromosome 2 |
| Location: | 4943635 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g103800 | (1 of 31) IPR003590 - Leucine-rich repeat, ribonuclease inhibitor subtype | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTTTGCTCCAGCCACTGCCCCGGCCGTC |
| Internal bar code: | ACCGGGCTGAGTCTCATAATTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 226 |
| LEAP-Seq percent confirming: | 99.6369 |
| LEAP-Seq n confirming: | 5488 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTAAGGGCATGGAGTGG |
| Suggested primer 2: | TTTATTGCTGTTGCCTGCTG |