| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.129386 |
| Chromosome: | chromosome 10 |
| Location: | 2516741 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g436450 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCACGGCGGCGCCCCAAGGGCCGCAAGGC |
| Internal bar code: | TAGCTATGCCTCTCCGGGCACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 866 |
| LEAP-Seq percent confirming: | 63.2091 |
| LEAP-Seq n confirming: | 390 |
| LEAP-Seq n nonconfirming: | 227 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTGTCTTGCACTCTTGCT |
| Suggested primer 2: | TCACGTACCATGCTGTCCAT |