| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.129389 |
| Chromosome: | chromosome 2 |
| Location: | 3232927 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095075 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCCCCGGGGCACTGAATCGAGCCGGGGCC |
| Internal bar code: | TTGGAGTCTTAGCTACCACTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 719 |
| LEAP-Seq percent confirming: | 99.0253 |
| LEAP-Seq n confirming: | 508 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCAACGAGTTGTTACGAGG |
| Suggested primer 2: | ACTAATGAACGTAACGCCCG |