| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.129406 |
| Chromosome: | chromosome 3 |
| Location: | 4617443 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g177150 | (1 of 2) K17263 - cullin-associated NEDD8-dissociated protein 1 (CAND1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTACCCGCTGCTGTACGGCCACCAGTTCC |
| Internal bar code: | CGGAAATGGGCGGCTTGGGCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 649 |
| LEAP-Seq percent confirming: | 28.5714 |
| LEAP-Seq n confirming: | 22 |
| LEAP-Seq n nonconfirming: | 55 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTAGCAGCCTTGTATGCGTG |
| Suggested primer 2: | CAGCTAGGATCGATTGCCTC |