| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.129412 |
| Chromosome: | chromosome 3 |
| Location: | 2057344 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g156550 | (1 of 1) PTHR22829//PTHR34826 - DEP DOMAIN PROTEIN // FAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCAAGCATGTGCAGCCCGCAAAAGCCAT |
| Internal bar code: | ATGAGGTTGGTCGCGATAATGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 203 |
| LEAP-Seq percent confirming: | 99.2275 |
| LEAP-Seq n confirming: | 1156 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTTGTCCTGTTGTCCCTCC |
| Suggested primer 2: | TCTGGGGCCATGACTAAGAC |