| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.129415 |
| Chromosome: | chromosome 12 |
| Location: | 9265995 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g541350 | (1 of 14) PF00566 - Rab-GTPase-TBC domain (RabGAP-TBC) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTGCAGAAAGGCACGCCTGCTTCCCTGC |
| Internal bar code: | TTCCGCGACGCCTGATTACCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 533 |
| LEAP-Seq percent confirming: | 96.9369 |
| LEAP-Seq n confirming: | 1076 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTCCGGGTTCCATTCCTAT |
| Suggested primer 2: | ACACACATGACCACCACCAC |