| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.129424 |
| Chromosome: | chromosome 1 |
| Location: | 3485965 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g022150 | KCN3 | Voltage-gated K+ channel; (1 of 1) PTHR11537//PTHR11537:SF158 - VOLTAGE-GATED POTASSIUM CHANNEL // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCCAAACAGATCGGCGGCAACTTCTCCA |
| Internal bar code: | CTTCTGTAAGTGTGCGTTTTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 278 |
| LEAP-Seq percent confirming: | 99.7048 |
| LEAP-Seq n confirming: | 3377 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGCAAACATGTACCCTGCC |
| Suggested primer 2: | ACTCCATACCGCTCACCATC |