| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.129440 |
| Chromosome: | chromosome 8 |
| Location: | 2233690 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g369900 | (1 of 2) IPR000104//IPR000719//IPR002290//IPR011009 - Antifreeze protein, type I // Protein kinase domain // Serine/threonine/dual specificity protein kinase, catalytic domain // Protein kinase-like domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCCACAGAGTGCCGGCGGCCCCACCAGC |
| Internal bar code: | GTTGATTTCTGTAAAGTTGAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 693 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTACTAACCCGCCTCTCG |
| Suggested primer 2: | GACCAAGCGAGTAGCAGTCC |