| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.129446 |
| Chromosome: | chromosome 13 |
| Location: | 3448781 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g587500 | AOF8 | (1 of 2) 1.3.5.5 - 15-cis-phytoene desaturase / Plant-type phytoene desaturase; Flavin-containing amine oxidase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAAAGAGGGGGCGTGTCATCCCTCTGGCC |
| Internal bar code: | TTTTTAGTTTACTGTAATCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 446 |
| LEAP-Seq percent confirming: | 99.5028 |
| LEAP-Seq n confirming: | 5003 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAAGCAAGTGCCAGTGCATC |
| Suggested primer 2: | CCTAAGTCTCCAAACGCAGC |