| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.129448 |
| Chromosome: | chromosome 3 |
| Location: | 4768790 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g178200 | CCT10 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGGCGTGCTGCGCCACCGCCGTGCCGTTA |
| Internal bar code: | CTGTAGTTCAGTGGTGGTGAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 322 |
| LEAP-Seq percent confirming: | 99.8645 |
| LEAP-Seq n confirming: | 737 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGTTGAGAGCAGCAATCC |
| Suggested primer 2: | ATGACATGACGAGCAAGCTG |