| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.129490 |
| Chromosome: | chromosome 12 |
| Location: | 5325830 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g529301 | (1 of 1) PTHR24096//PTHR24096:SF190 - FAMILY NOT NAMED // EBONY | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCTGCAAGCTTTAGTGCAGTGCGCCTCC |
| Internal bar code: | GCAGAAATTGGCGTAACGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 829 |
| LEAP-Seq percent confirming: | 98.447 |
| LEAP-Seq n confirming: | 1775 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGGTTATGATGGGTCAGG |
| Suggested primer 2: | TCCTGCAGCCATCACAGTAG |