| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.129502 |
| Chromosome: | chromosome 9 |
| Location: | 2794615 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g389001 | (1 of 77) IPR001623 - DnaJ domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGCGGCCCGCTCCGGATTCGGGTGCTTT |
| Internal bar code: | GTGGGTTGGGGGCTAAAAAAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 187 |
| LEAP-Seq percent confirming: | 46.3595 |
| LEAP-Seq n confirming: | 2445 |
| LEAP-Seq n nonconfirming: | 2829 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGTGGATGTGGAGTCCTT |
| Suggested primer 2: | ACACGGTACACGAATGGGAT |