| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.129507 |
| Chromosome: | chromosome 2 |
| Location: | 2196697 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g089650 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATCTCACCTGCAATGGCCCCGGCCCCCGC |
| Internal bar code: | GATCAGCGTCGTTTAGGTTAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 310 |
| LEAP-Seq percent confirming: | 76.8407 |
| LEAP-Seq n confirming: | 574 |
| LEAP-Seq n nonconfirming: | 173 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGGTAGAACTCCTCCTCGT |
| Suggested primer 2: | CGCGCTTCCAGAATTTAGAC |