Insertion junction: LMJ.RY0402.129510_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.129510
Chromosome:chromosome 12
Location:4862259
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre12.g525100 SMC6B (1 of 2) PTHR19306//PTHR19306:SF6 - STRUCTURAL MAINTENANCE OF CHROMOSOMES 5,6 SMC5, SMC6 // PROTEIN C23H4.6, ISOFORM A-RELATED; Structural Maintenance of Chromosomes protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTCGTCCTGGCACATGCTCTGACTGTGCC
Internal bar code:CATGGAATCATCGTCGGAACGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:299
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:342
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GCCGTAATTGCTGGTGTTTT
Suggested primer 2:AGATACCATGCTTCCCATGC