Insertion junction: LMJ.RY0402.129522_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.129522
Chromosome:chromosome 9
Location:6945560
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g410500 PAP11 (1 of 1) IPR002934//IPR023214 - Polymerase, nucleotidyl transferase domain // HAD-like domain; Class-II RNA nucleotidyl transferase 11 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCATCGTATTCGTCTTCACACTCGTCATC
Internal bar code:GCGGGATAAAGAGTTTAAAATC

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:0.0
LEAP-Seq n confirming:0
LEAP-Seq n nonconfirming:1150
LEAP-Seq n unique pos:0

Suggested primers for confirmation by PCR

Suggested primer 1:GGCTCAGCTGTCGTACCTTC
Suggested primer 2:TTCCGTCACAGTCTCACAGC