Insertion junction: LMJ.RY0402.129531_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.129531
Chromosome:chromosome 5
Location:3196399
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre05.g239750 ARS13 (1 of 2) IPR002591//IPR017849//IPR017850 - Type I phosphodiesterase/nucleotide pyrophosphatase/phosphate transferase // Alkaline phosphatase-like, alpha/beta/alpha // Alkaline-phosphatase-like, core domain; Arylsulfatase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCTGTTTCCCAACTTCTTTCTGTCCCTAC
Internal bar code:TAGGCGATCTTGCGGACTGCCG

Confirmation - LEAP-Seq

LEAP-Seq distance:939
LEAP-Seq percent confirming:98.9657
LEAP-Seq n confirming:2392
LEAP-Seq n nonconfirming:25
LEAP-Seq n unique pos:25

Suggested primers for confirmation by PCR

Suggested primer 1:GTACCCGAAATGTGACACCC
Suggested primer 2:CAGGAGGCGAAAGTAACGAG