Insertion junction: LMJ.RY0402.129531_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.129531
Chromosome:chromosome 5
Location:3196400
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre05.g239750 ARS13 (1 of 2) IPR002591//IPR017849//IPR017850 - Type I phosphodiesterase/nucleotide pyrophosphatase/phosphate transferase // Alkaline phosphatase-like, alpha/beta/alpha // Alkaline-phosphatase-like, core domain; Arylsulfatase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGAGATATCAGCGCACCCAACGGCAGAGG
Internal bar code:GCGTCTCTGTACGACCAGGGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:434
LEAP-Seq percent confirming:99.738
LEAP-Seq n confirming:6090
LEAP-Seq n nonconfirming:16
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GTACCCGAAATGTGACACCC
Suggested primer 2:CAGGAGGCGAAAGTAACGAG