| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.129539 |
| Chromosome: | chromosome 3 |
| Location: | 3112343 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g164900 | (1 of 1) PTHR24361//PTHR24361:SF159 - MITOGEN-ACTIVATED KINASE KINASE KINASE // SERINE/THREONINE-PROTEIN KINASE OSR1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCAGTTCTTCTGACAAACTACATGCATC |
| Internal bar code: | GGTAGATAAATGTCATAGGGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 725 |
| LEAP-Seq percent confirming: | 99.3687 |
| LEAP-Seq n confirming: | 787 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTTTCGCTCTTGTCACACA |
| Suggested primer 2: | GTTTGTTGGTTGCTCGGAAT |