| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.129541 |
| Chromosome: | chromosome 9 |
| Location: | 919313 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g401100 | (1 of 1) PTHR12560//PTHR12560:SF0 - LONGEVITY ASSURANCE FACTOR 1 LAG1 // LD18904P | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTACCACTAGCAGCGGGAGTGCTGCCGCG |
| Internal bar code: | GGCCCGCACCCCGCGAAGCTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 975 |
| LEAP-Seq percent confirming: | 99.7775 |
| LEAP-Seq n confirming: | 4485 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCGTTCCCAAGTACGCAAT |
| Suggested primer 2: | CTTGAGGACTGAGGTAGCCG |