| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.129543 |
| Chromosome: | chromosome 1 |
| Location: | 4896498 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g033950 | EXN2 | 3'-5' exonuclease with GRF zinc finger domain; (1 of 1) K18417 - ERI1 exoribonuclease 2 [EC:3.1.-.-] (ERI2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGGGAAAGGACACAACACACGCCACGGT |
| Internal bar code: | GATTTTTCTGTGATCTTTTGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 368 |
| LEAP-Seq percent confirming: | 99.8869 |
| LEAP-Seq n confirming: | 883 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCTGCTCCTCTCGTTGTC |
| Suggested primer 2: | ATTACAGGTCTTTGGTGCCG |