Insertion junction: LMJ.RY0402.129547_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.129547
Chromosome:chromosome 15
Location:1295585
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre15.g641750 (1 of 3) PF05495 - CHY zinc finger (zf-CHY) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTGGTTTGATCGTGCAGAGACACAGGCGA
Internal bar code:CGATCTGCTTTTGATTAATCTC

Confirmation - LEAP-Seq

LEAP-Seq distance:88
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TTTTGCTAGCCACGAGGTTT
Suggested primer 2:AGCAAGCGGCTGATGTAGTT