Insertion junction: LMJ.RY0402.129551_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.129551
Chromosome:chromosome 2
Location:1514850
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g084250 PPP7 (1 of 2) PTHR13832//PTHR13832:SF267 - PROTEIN PHOSPHATASE 2C // PROTEIN PHOSPHATASE 1K, MITOCHONDRIAL; Phosphoprotein phosphatase 2C-related 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGTTGGCTCGCACTCGGCGGCCGCTACGC
Internal bar code:TGCCTTGGGGCCGGACCGACTA

Confirmation - LEAP-Seq

LEAP-Seq distance:403
LEAP-Seq percent confirming:99.7856
LEAP-Seq n confirming:931
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:AGAGGTTCGAAGCAACGTGT
Suggested primer 2:CCCTTTCCGACTGTCAACAT