| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.129554 |
| Chromosome: | chromosome 1 |
| Location: | 5731181 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g040850 | (1 of 1) IPR002048//IPR003368//IPR006626//IPR010308//IPR011050 - EF-hand domain // Polymorphic outer membrane protein repeat // Parallel beta-helix repeat // TRP-like family // Pectin lyase fold/virulence factor | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGCCAGGGCAGTGCACGGAGGCTGGGAG |
| Internal bar code: | GCGTCAAGCGAGGGGTACGTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 77 |
| LEAP-Seq percent confirming: | 89.011 |
| LEAP-Seq n confirming: | 81 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGTAACCCAGACCGAAGAC |
| Suggested primer 2: | TTACTTTGGTCCATCTCGGC |