Insertion junction: LMJ.RY0402.129557_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.129557
Chromosome:chromosome 11
Location:3003259
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre11.g478456 (1 of 45) IPR029052 - Metallo-dependent phosphatase-like intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACCAGCTGGTAACCCTCTCCCTCTCCCTC
Internal bar code:CTAGGGGAGCCGGCGAACAAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:613
LEAP-Seq percent confirming:73.6276
LEAP-Seq n confirming:3836
LEAP-Seq n nonconfirming:1374
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:CTCCCTTCTTTGTGACGAGC
Suggested primer 2:TAACTGCTGCTGTCAGTGCC