Insertion junction: LMJ.RY0402.129557_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.129557
Chromosome:chromosome 11
Location:3003319
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre11.g478456 (1 of 45) IPR029052 - Metallo-dependent phosphatase-like intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGGTCTTAGGGTGGTGTCCGCGCCCCTTT
Internal bar code:GCCACCCGCACACCCTAGACCT

Confirmation - LEAP-Seq

LEAP-Seq distance:856
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:221
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CTCCCTTCTTTGTGACGAGC
Suggested primer 2:TAACTGCTGCTGTCAGTGCC