| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.129567 |
| Chromosome: | chromosome_6 |
| Location: | 6310630 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g291700 | RSP3 | Radial spoke protein 3 | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AGCGACCTCTCCCCCAATCCACTCCGTACC |
| Internal bar code: | ACAGACCCTTCGGCGGCCTATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 739 |
| LEAP-Seq percent confirming: | 99.3902 |
| LEAP-Seq n confirming: | 5542 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGCCTAAGGTTGTCGAGC |
| Suggested primer 2: | ACAAGGCCTACACCAACCAC |