Insertion junction: LMJ.RY0402.129571_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.129571
Chromosome:chromosome 9
Location:4204609
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g394251 PF16 Central pair associated protein; (1 of 1) PF00514//PF13513 - Armadillo/beta-catenin-like repeat (Arm) // HEAT-like repeat (HEAT_EZ) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTCTGATGTAAAATACTCAAGTGAACAGA
Internal bar code:CGTTGTGCTCCTACGCGGAACT

Confirmation - LEAP-Seq

LEAP-Seq distance:797
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:296
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GATGTGTCAGCGACGAAAGA
Suggested primer 2:ACAACTCGAAGTGGTTTGGG