Insertion junction: LMJ.RY0402.129575_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.129575
Chromosome:chromosome 1
Location:3575301
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre01.g022950 (1 of 8) PTHR26379:SF144 - BTB/POZ DOMAIN-CONTAINING PROTEIN 19 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAGTCATCCTTGGGGTCTTGCCAATTGCGC
Internal bar code:CGAGTCCGTGTTGGTGGTACTG

Confirmation - LEAP-Seq

LEAP-Seq distance:782
LEAP-Seq percent confirming:97.8261
LEAP-Seq n confirming:90
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:TTGGACCTGGAATACTTGCC
Suggested primer 2:AGAACTTCTTGCACCACGCT