| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.129586 |
| Chromosome: | chromosome 8 |
| Location: | 2003501 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g368050 | MGMT | Methylated-DNA--[protein]-cysteine S-methyltransferase; (1 of 1) 2.1.1.63 - Methylated-DNA--[protein]-cysteine S-methyltransferase / O-6-methylguanine-DNA-alkyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGCACACGTGCTTGCTCAGGTGCACCTG |
| Internal bar code: | ACGAGCTCGGTTACATATTTCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 912 |
| LEAP-Seq percent confirming: | 99.5451 |
| LEAP-Seq n confirming: | 3939 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCTACCCTACACCTGCACC |
| Suggested primer 2: | ACAGCGTTTGAGAACAGGCT |