| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.129596 |
| Chromosome: | chromosome 14 |
| Location: | 1039107 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g614450 | Hypothetical protein; (1 of 781) IPR000104 - Antifreeze protein, type I | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGAAGCTGTGCTTGAATGCTGCATCGTC |
| Internal bar code: | ATAAATTCAGAGGAGGCGCCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 883 |
| LEAP-Seq percent confirming: | 99.3247 |
| LEAP-Seq n confirming: | 1765 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTTGGTTACGAGGGCACAG |
| Suggested primer 2: | TGCATACATCCCTGTCGGTA |