| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.129600 |
| Chromosome: | chromosome 2 |
| Location: | 3203049 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095071 | (1 of 2) K01148 - poly(A)-specific ribonuclease (PARN) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGAGGGGAACACAGCTTTGGGATGCGTGT |
| Internal bar code: | GCTCGCGTTCGATGATTGACCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 386 |
| LEAP-Seq percent confirming: | 98.7382 |
| LEAP-Seq n confirming: | 939 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCTACGACGCCTACATGA |
| Suggested primer 2: | GGGGTTACGGGTTAGGTTGT |