| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.129660 |
| Chromosome: | chromosome 8 |
| Location: | 4324346 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g381450 | OPR35 | OctotricoPeptide Repeat protein 35 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTTGCCGGTGAGCTCGGCGCAGCACAGCG |
| Internal bar code: | GGGTCACGACATCCGCTTAACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 541 |
| LEAP-Seq percent confirming: | 98.8235 |
| LEAP-Seq n confirming: | 84 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGGAGGAGGAGGAAGAGA |
| Suggested primer 2: | AACATATGGGTAGGGGGAGC |