| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.129679 |
| Chromosome: | chromosome 10 |
| Location: | 165944 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g418900 | (1 of 18) K13412 - calcium-dependent protein kinase (CPK) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTCCTGTTGCAGGCACTGCTGTCTCGTGT |
| Internal bar code: | AGAAAGATTCCGGAAGGTTGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 610 |
| LEAP-Seq percent confirming: | 92.8783 |
| LEAP-Seq n confirming: | 2191 |
| LEAP-Seq n nonconfirming: | 168 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTCCACGAGTATGTCGCAG |
| Suggested primer 2: | GTTGCTTGTGCTGCTTGTGT |