| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.129716 |
| Chromosome: | chromosome 1 |
| Location: | 5461164 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g038400 | CRT2 | Calreticulin 2, calcium-binding protein; (1 of 1) K08057 - calreticulin (CALR) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCGCTTCTAACGCGTCCGATCTAACATCA |
| Internal bar code: | GGGGGTGTAGCTAAGGACCCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 300 |
| LEAP-Seq percent confirming: | 99.5836 |
| LEAP-Seq n confirming: | 1435 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGCACAAGGACGAGGAGAA |
| Suggested primer 2: | ATTCCTCCGAGACGACACAC |