| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.129720 |
| Chromosome: | chromosome 8 |
| Location: | 4512783 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g382590 | (1 of 1) K11662 - actin-related protein 6 (ACTR6, ARP6) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTGAGTTATGAGGCGAACTACATAAACTG |
| Internal bar code: | GCATTCGTAGAATCACACTACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 683 |
| LEAP-Seq percent confirming: | 95.4098 |
| LEAP-Seq n confirming: | 291 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGAGTTTGGCGCTCTGTCT |
| Suggested primer 2: | AACCCCTTCCCTCATACACC |