Insertion junction: LMJ.RY0402.129777_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.129777
Chromosome:chromosome 12
Location:4463104
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre12.g521500 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCACACCAATGACCCCACTTTGCCAACCT
Internal bar code:CGCAGCGTGTTAGCCCGCTTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:882
LEAP-Seq percent confirming:99.7833
LEAP-Seq n confirming:1842
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:CGCTACTGCAAACGTGTGTT
Suggested primer 2:CAATCAACGGTGTGTTCAGG