| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.129787 |
| Chromosome: | chromosome 16 |
| Location: | 4863915 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g686000 | SRS15 | (1 of 1) K13096 - splicing factor 4 (SF4); putative RNA-binding protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCTGGGCAACCCGCGCAGGGCCTACTAC |
| Internal bar code: | GGAGGGCAAGGGCCTCGCAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 382 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 58 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTACAGGAGGGTTTGGCAC |
| Suggested primer 2: | GTCCACTGCCACATCATACG |