| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.129804 |
| Chromosome: | chromosome 12 |
| Location: | 9134103 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g542550 | (1 of 13) 2.3.1.225 - Protein S-acyltransferase / S-protein acyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGTTGTGGGAGTGGCGAGTGGTGGTGAG |
| Internal bar code: | TGGAAAAGACAGCTCTTCGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 405 |
| LEAP-Seq percent confirming: | 99.4464 |
| LEAP-Seq n confirming: | 2874 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAGCGAGAACCCGTTGAAG |
| Suggested primer 2: | TGACTAGGGCTGACAATCCC |