Insertion junction: LMJ.RY0402.129807_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.129807
Chromosome:chromosome 3
Location:8712020
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g204465 GT90F36,GT90-36 GT90 family protein 36; (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTTGATCATCAGCCGAACCACCTTTCCGTT
Internal bar code:GCATCTTGGCTGGTTCGCAGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:1069
LEAP-Seq percent confirming:99.4796
LEAP-Seq n confirming:3441
LEAP-Seq n nonconfirming:18
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:AGAGGGACCTGTCATGGTTG
Suggested primer 2:CGCAAGTAGGTGGGAGGATA