| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.129816 |
| Chromosome: | chromosome 2 |
| Location: | 4403111 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g099750 | (1 of 112) 2.7.11.25 - Mitogen-activated protein kinase kinase kinase / MLTK | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGAGGTCACACCGTCAAACCCATTTGCCT |
| Internal bar code: | GGGTAAGTGCACCTGGGGATGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 909 |
| LEAP-Seq percent confirming: | 99.6096 |
| LEAP-Seq n confirming: | 5613 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGTCCCGTAAAAGTCCCGT |
| Suggested primer 2: | CGCTTACATTTGGTGGTGTG |