| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.129847 |
| Chromosome: | chromosome 6 |
| Location: | 5505140 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g284850 | (1 of 1) K03155 - timeless (TIMELESS) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCACAACACAACACACCCCAGAGCCAAT |
| Internal bar code: | TCGCAGGGCCCACCCGTGTTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 319 |
| LEAP-Seq percent confirming: | 99.6536 |
| LEAP-Seq n confirming: | 863 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAGCTCCTCATGGAGGTAA |
| Suggested primer 2: | AAGCGCGTAAGGATTAAGCA |