| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.129850 |
| Chromosome: | chromosome 3 |
| Location: | 6597284 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g196350 | (1 of 3) K09858 - SEC-C motif domain protein (K09858) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGCGCACAAACCCCCACTATGACGTTGGG |
| Internal bar code: | ACTGTAGCGAGTCCGTTGCTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 757 |
| LEAP-Seq percent confirming: | 99.7934 |
| LEAP-Seq n confirming: | 5797 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGAGCTCCTGCACCAGTGT |
| Suggested primer 2: | CAAGGCTCATCAACTGCAAA |