| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.129859 |
| Chromosome: | chromosome_1 |
| Location: | 5496009 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g038750 | MOT7 | Predicted protein | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGCAGACGAGGGTGGTCTGCGTCGCTTTTA |
| Internal bar code: | AGGTCTATCATTTGCTTGCTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 306 |
| LEAP-Seq percent confirming: | 99.7773 |
| LEAP-Seq n confirming: | 448 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCGTAGAGAAGCCAGGAT |
| Suggested primer 2: | TTGTGCGTGCTACGCTAATC |