| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.129910 |
| Chromosome: | chromosome 12 |
| Location: | 4757945 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g524000 | SUC1,GTR7 | (1 of 1) 2.4.1.14 - Sucrose-phosphate synthase / UDP-glucose-fructose-phosphate glucosyltransferase; Sucrose-phosphate synthase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGAGCAGTGGGGGCTGTACGACGGGTGC |
| Internal bar code: | ACGAACCGATGGCCGGGCGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 260 |
| LEAP-Seq percent confirming: | 97.8329 |
| LEAP-Seq n confirming: | 4695 |
| LEAP-Seq n nonconfirming: | 104 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTACCTCAAGTGAGCAGGGC |
| Suggested primer 2: | CCCTAACAACCCCTAGCTCC |